Search results for "water channel"

showing 3 items of 3 documents

The inhibition of glycerol permeation through aquaglyceroporin-3 induced by mercury(II)

2016

Mercurial compounds are known to inhibit water permeation through aquaporins (AQPs). Although in the last years some hypotheses were proposed, the exact mechanism of inhibition is still an open question and even less is known about the inhibition of the glycerol permeation through aquaglyceroporins. Molecular dynamics (MD) simulations of human aquaporin-3 (AQP3) have been performed up to 200 ns in the presence of Hg2+ ions. For the first time, we have observed the unbiased passage of a glycerol molecule from the extracellular to cytosolic side. Moreover, the presence of Hg2+ ions covalently bound to Cys40 leads to a collapse of the aromatic/arginine selectivity filter (ar/R SF), blocking th…

Glycerol0301 basic medicineMolecular dynamicCell Membrane PermeabilityBiochemistryProtein Structure Secondarychemistry.chemical_compoundGLPFCOORDINATIONCRYSTALEscherichia coli ProteinsPermeationBiochemistryCovalent bondSettore CHIM/03 - Chimica Generale E InorganicaPhosphatidylcholinesCOMPLEXESProtein BindingSTRUCTURAL BASISCations DivalentPlasmodium falciparumAquaporinCYSTEINE-189Molecular Dynamics SimulationMolecular dynamicsAquaporinsWATER CHANNELInorganic Chemistry03 medical and health sciencesEscherichia coliGlycerolExtracellularHumansMoleculePERMEABILITYProtein Structure QuaternaryAquaporin 3Binding SitesAQUAPORIN INHIBITIONWaterBiological TransportMembranes ArtificialAquaglyceroporinMercurySIMULATIONSProtein Structure TertiaryCytosolWater permeation030104 developmental biologyAquaglyceroporinschemistryStructural Homology ProteinBiophysicsGlycerol permeationJournal of Inorganic Biochemistry
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Expression of PIP1 and PIP2 aquaporins is enhanced in olive dwarf genotypes and is related to root and leaf hydraulic conductance

2007

The expression of OePIP1.1 and OePIP2.1 aquaporins in root, stem and leaf was higher in Olea europaea L. (olive) plants carrying a dwarfing (D) clone as rootstock, than in plants carrying a vigorous (M) clone as rootstock. The highest difference of expression between plants with D and M rootstocks was detected in the root and for the PIP2 gene, the transcripts of which show a high water channel activity in oocytes.  Compared with plants with M rootstock, plants with D rootstock showed reduced root and canopy biomass and reduced hydraulic conductance of the bulk root system (Kroot). Hydraulic conductance of D roots was higher than that of M roots when Kroot was scaled by root DW, in agreemen…

Canopyhydraulic conductancePhysiologyshootAquaporindroughtPlant ScienceRoot systemembolismAquaporin; water channel; cavitation; drought; embolism; olive; hydraulic conductance; shoot; water stress; compensatory mechanism.olivewater stresscavitationGene expressionBotanyGeneticsbiologyAquaporinfungifood and beverageswater channelCell BiologyGeneral Medicinebiology.organism_classificationGraftingDwarfingcompensatory mechanismOleaRootstockPhysiologia Plantarum
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Short-term response to waterlogging in Quercus petraea and Quercus robur: A study of the root hydraulic responses and the transcriptional pattern of …

2015

International audience; We characterized the short-term response to waterlogging in Quercus petraea (Matt.) Liebl. and Quercus robur L. as the initial response towards their known long-term differences in tolerance to waterlogging. One-month old seedlings were subjected to hypoxic stress and leaf gas exchange, shoot water potential (Psi(s)) and root hydraulic conductivity (Lp(r)) were measured. In parallel, the expression of nine aquaporins (AQPs) along the primary root was analysed by quantitative RT-PCR. Results showed a similar reduction in net assimilation (A) and stomatal conductance (g(s)) for the two species. Notably, the response of Lpr differed temporally between the two species. Q…

Stomatal conductanceTime FactorsTranscription GeneticPhysiology[SDE.MCG]Environmental Sciences/Global ChangesAquaporinPlant ScienceAquaporinsPlant RootsQuercus roburQuercusHydraulic conductivityGene Expression Regulation PlantBotanyGeneticsHypoxiaQuercus robur (pedunculate oak)Plant ProteinsQuercus petraea (sessile oak)Water transportbiologyWaterWater channelbiology.organism_classificationOxygenPlant Leaves[ SDE.MCG ] Environmental Sciences/Global ChangesShootQuercus petraeaStress conditionsRoot hydraulic conductivityPlant ShootsPlant Physiology and Biochemistry
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